Figure 4.

Sirt3 overexpression protects neuronal viability and mitochondrial function by activating the UPR^mt. WT and Sirt3^Tg mice were subjected to transient MCAO. N2a cells were transfected with Ad-Ctrl or Ad-Sirt3, and then were subjected to OGD/R to simulate cerebral I/R injury in vitro. A-D. RNA was collected from brain tissues, and mtDNAj, Lonp1, ClpP and Hsp10 levels were measured using qPCR. E-H. The mRNA levels of mtDNAj, Lonp1, ClpP and Hsp10 were evaluated using qRT-PCR in N2a cells subjected to OGD/R. I. AEBSF was used to inhibit the UPR^mt, and cell viability was determined with an MTT assay. J. AEBSF was used to inhibit the UPR^mt, and then an LDH release assay was used to measure LDH levels in media from N2a cells. K. AEBSF was used to inhibit the UPR^mt, and then the concentration of ATP in N2a cells was detected with an ELISA. *p<0.05 vs. Ad-Ctrl group or sham+WT group, #p<0.05 vs. OGD/R+Ad-Ctrl group or MCAO+WT group, @p<0.05 vs. OGD/R+Ad-Sirt3 group.