Figure 5.

Sirt3 activates the UPR^mt in neurons through the Foxo3a/Sphk1 pathway. WT and Sirt3^Tg mice were subjected to transient MCAO. N2a cells were transfected with Ad-Ctrl or Ad-Sirt3, and then were subjected to OGD/R to simulate cerebral I/R injury in vitro. A-C. Proteins were isolated from brain tissues, and Western blotting was used to measure the expression of Foxo3 and Sphk1. D-F. Immunofluorescence was used to observe changes in Foxo3 and Sphk1 levels in N2a cells after OGD/R. G-J. Carbenoxolone (CBX) was used to inhibit the Foxo3/Sphk1 pathway, and then qRT-PCR was performed to measure mtDNAj, Lonp1, ClpP and Hsp10 mRNA levels in N2a cells subjected to OGD/R. *p<0.05 vs. Ad-Ctrl group or sham+WT group, #p<0.05 vs. OGD/R+Ad-Ctrl group or MCAO+WT group, @p<0.05 vs. OGD/R+Ad-Sirt3 group.