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. 2023 Aug 15;19(13):4259–4277. doi: 10.7150/ijbs.85986

Figure 6.

Figure 6

Overexpression of PAI-1 in B16F10-C3 cells enhanced cell survival and metastasis. (A) Average FPKM value of SERPINE1 encoding PAI-1 in monocultured and cocultured B16F10-C3 and 231-GFP cells. (B and C) The mRNA and protein levels of PAI-1 in the EV and OE-PAI-1 groups. (D) Representative IF images of PAI-1 distribution. Scale bar, 20 μm. (E and F) Representative contrast images and quantification of cell viability between EV and OE-PAI-1 cells. Scale bar, 100 μm. (G and H) Fluorescent images and colony count in left lung 5 h post tail vein injection of EV and OE-PAI-1 cells (n = 6). Scale bar, 1 mm. (I to K) Images and number of migrated and invaded EV and OE-PAI-1 cells. Scale bar, 200 μm. (L and M) Images and quantification of the colony formation assay for the EV and OE-PAI-1 cells. Scale bar, 5 mm. (N and O) Fluorescent images and colony count after overexpression of PAI-1 21 days post injection through the tail vein (n = 6 to 8). Scale bar, 1 mm. (P) H&E staining results of the mouse lung lobe. Scale bar, 20 μm. The results are displayed as the mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001.