TABLE 3.
Culture results for statistically significant concentrations of CB-18 (P < 0.05)
| Critical parameter for isolate | Time [days] to positive result [GI ≥ 15] (CB-18 concn [μg/ml])
|
Time [days] to max GI [999] (CB-18 concn [μg/ml])
|
CB-18 concn [μg/ml] with no growth
|
|||
|---|---|---|---|---|---|---|
| 12B/RF | 12B/PANTA/caz | 12B/RF | 12B/PANTA/caz | 12B/RF | 12B/PANTA/caz | |
| M. tuberculosis ATCC 27294 | ||||||
| Control | 8.8 ± 0.5a | 10.2 ± 0.5 | 13.5 ± 1.0 | 16.8 ± 0.5 | ||
| Time at significant concn of CB-18 | >35 | 35 | ||||
| First | 10.5 ± 0.6b (20)c | 12.5 ± 1.0 (20) | 17.5 ± 0.6 (20) | 18.8 ± 0.5 (10) | ||
| Second | 12.5 ± 1.0d (30)e | 15.0 ± 1.6 (25) | 21.8 ± 2.5 (25) | >28 (20) | ||
| M. tuberculosis 571/573-S1 | ||||||
| Control | 9.2 ± 0.5 | 10.8 ± 1.0 | 15.0 ± 0.0 | 16.0 ± 0.8 | ||
| Time at significant concn of CB-18 | 25 | 25 | ||||
| First | 11.0 ± 0.8 (10) | 12.5 ± 1.0 (10) | >28 (20) | 17.8 ± 0.5 (5) | ||
| Second | 18.2 ± 1.0 (20) | >28 (20)f | >28 (25) | >28 (20) | ||
| M. avium ATCC 25291 | ||||||
| Control | 6.0 ± 0.0 | 8.8 ± 1.0 | 10.2 ± 0.5 | 16.8 ± 2.1 | ||
| Time at significant concn of CB-18 | >35 | >35 | ||||
| First | 7.5 ± 0.6 (30) | 11.8 ± 1.0 (15) | 11.2 ± 0.5 (15) | 21.0 ± 2.4 (15) | ||
| Second | Not tested (>35) | 16.0 ± 2.7 (20) | 13.8 ± 1.0 (20) | >28 (25) | ||
| M. kansasii ATCC 12478 | ||||||
| Control | 3.0 ± 0.0 | 4.5 ± 0.6 | 7.0 ± 0.0 | 8.8 ± 0.5 | ||
| Time at significant concn of CB-18 | 30 | >35 | ||||
| First | 4.5 ± 0.6 (20) | 5.8 ± 0.3 (25) | >28 (25)g | 24.0 ± 2.0 (20) | ||
| Second | 6.2 ± 0.5 (25) | Not tested (>35) | >28 (30) | >28 (25) | ||
| M. fortuitum ATCC 6841 | ||||||
| Control | 1.0 ± 0.0 | 1.0 ± 0.0 | 2.0 ± 0.0 | 3.2 ± 0.5 | ||
| Time at significant concn of CB-18 | >100 | >100 | ||||
| First | 2.5 ± 0.6 (30) | 6.8 ± 1.7 (30) | 4.8 ± 1.7 (30) | 5.0 ± 0.8 (10) | ||
| Second | 7.2 ± 0.5 (100) | 10.0 ± 1.4 (40) | 8.8 ± 1.5 (40) | 10.5 ± 1.7 (30) | ||
This is the average time (± standard deviation) to a given result in the absence of CB-18.
This time represents the average time (± standard deviation) to a given result at the lowest CB-18 concentration at which the first statistically significant change occurred.
This is the CB-18 concentration at which the first statistically significant change occurred for a given result relative to the control result.
This time represents the average time (± standard deviation) to a given result at the second statistically significant CB-18 concentration.
This concentration represents the next, or second, statistically significant change that occurred relative to the CB-18 concentration at which the first statistically significant change occurred.
Although the graph of this result appears to be positive, only one of the four replicates became positive during the 4-week incubation.
The time to maximum GI result at 20 μg/ml (17.8 ± 8.8 days) was not significantly different (P = 0.081) from the control result because of the wide replicate-to-replicate variation in this series of bottles.