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Design and validation of sgRNAs for CRISPR/Cas9-mediated mutagenesis. (A-D) Diagrams of selected candidate gene loci and indel analysis plot for each selected sgRNA, based on next-generation sequencing of amplicons. Blue arrows indicate CRISPR/Cas9-induced indel peak, red asterisks indicate naturally-occurring indels. Blue sgRNA identifiers indicate top sgRNAs selected for phenotypic assay. Grey identifiers indicate sgRNA designed, tested, but not selected for further use.
