Fig. 4.

Heritability of genome editing mutations to progenies. (A) The number, genotype and sex of G1 mice of eight inbred strains are shown. The outer layer represents sex (blue for male and red for female), the middle layer represents the allele carried by each G1 mouse, and the inner layer shows G0 male mice used for each strain. Unread* alleles might include potential wild-type, inversion or large rearrangement alleles (not confirmed). The presence of these alleles was confirmed by electrophoresis of genomic PCR products. Note that all biological mothers of G1 mice were wild type. (B) Read percentages of mutant alleles harboured by each father of G1 mice based on DAJIN analysis. The bar colour links to the colour of each allele in G1 mice in A. Dark grey indicates unread* alleles that were not selected for detailed analysis in the genotyping of G1 mice.