FIGURE 2.

The expression of FSP1 affects sorafenib-induced ferroptosis. (A–F) The specified plasmids or short interfering RNAs were transiently transfected into HCC cells, which were subsequently treated with sorafenib or without sorafenib (10 μM, 36 h). The levels of the indicated proteins (A); cell viability (B); MDA levels (C); GSH levels (D); C11-BODIPY flow cytometry analysis (E); and C11-BODIPY stain (× 400 magnification) (F) were assayed. The data are the means ± SD of the 3 independent experiments. *p < 0.05, **p < 0.01, and ***p < 0.001. Abbreviation: FSP1, ferroptosis suppressor protein 1.