Figure S1.

HuR expression in NK cells and steady state phenotype of HuR CKO NK cells. (A) Expression of HuR in NK cells in response to stimulation with plate-bound antibody or indicated cytokines for 1 d. Representative of two experiments in triplicate. (B) HuR expression by splenic lymphocytes in HuR CKO and littermate control mice. Filled histograms show HuR staining and open histograms indicate isotype control. Representative of two independent experiments. (C) Peripheral blood and splenic NK cell numbers in HuR CKO and littermates analyzed by flow cytometry. Cumulative of two experiments totaling four to six mice per group. (D) Maturation of splenic NK cells measured by CD27 and CD11b staining. (E) Receptor repertoire on splenic NK cells in HuR CKO and littermate control mice. Cumulative of two experiments totaling four to six mice per group. Statistics were calculated using unpaired t tests with Bonferroni correction. Error bars indicate SEM; ns: not significant, *P < 0.05, **P < 0.01, ***P < 0.001, and ****P < 0.0001. MFI, median fluorescent intensity.