Figure S2.

HuR expression and function of HuR-deficient NK cells during MCMV infection. (A) HuR expression by splenic C57BL/6 NK cells at indicated day after infection, analyzed by flow cytometry. Representative of two independent experiments with three mice per time point. (B) Number of splenic NK cells in Ncr1 cre/wt × HuR fl/wt and littermate Ncr1 wt/wt × HuR fl/wt control mice at 7 d after MCMV infection. (C) Competitive expansion of HuR CKO (left) or littermate control with congenic CD45.1 WT NK cells transferred into Ly49H-deficient hosts and subsequently infected with MCMV. Representative of two independent experiments with three to five mice per group. (D) Analysis of MCMV m157 sequences in spleens of RAG HuR CKO and RAG littermate mice that succumbed to MCMV infection. Cumulative of two independent experiments totaling nine mice per group with 10 m157 sequences analyzed per mouse. Statistics were calculated using unpaired t tests with Bonferroni correction. Error bars indicate SEM; ns: not significant, *P < 0.05, and **P < 0.01. MFI, median fluorescent intensity.