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. 2023 Jan 26;41(9):1307–1319. doi: 10.1038/s41587-022-01651-1

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Extended Data Fig. 1 — Axial cross sections through support are shown for a) wide-field, b) 3D SIM, c) standing wave, d) I⁵S, and e) four-beam, mirror-based SIM systems. Dots indicate illumination spatial frequency components, with green dots in d, e) indicating additional frequency components that are not present in 3D SIM. Supports were simulated with the following parameters: emission NA = 1.35; refractive index of immersion medium = 1.406; excitation wavelength: 488 nm; emission wavelength: 525 nm; side beams in b, d, e) at 92% of pupil radius.