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. 2023 Jan 26;41(9):1307–1319. doi: 10.1038/s41587-022-01651-1

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Extended Data Fig. 2 — a) Photograph of mounting scheme, indicating hardware mounts with vendor information. PI: Physik Instrumente. b) Schematic to accompany a), showing kinematic mirror mount, Piezo Z scanner, mirror, sample, and objective lens. Schematic is not to scale. c) Alignment of the reflected beam is achieved by manually adjusting the knobs on the kinematic mirror mount until tilt is minimized. This can be achieved by monitoring the autofluorescence from a dirty coverslip immersed in liquid; tilt is minimized when a flat intensity profile is achieved when translating the sample through focus, yielding a purely axial modulation. Top images show reflected beam at different tilts of mirror; bottom images show corresponding planar images of autofluorescence when lateral modulation is present (left, middle) versus mostly axial modulation (right). See also Supplementary Video 1 and Methods for more information.