Table 1.
The guide RNA sequences designed to alter the AR sequence and efficacy of editing.
| Guide | Guide RNA sequence (5′ to 3′) | Exon | Mutation ratea after microinjection | Mutation ratea after electroporation |
|---|---|---|---|---|
| AR_2A | GCACCTCGAAAGGTCTTGGA | 2 | 3/3 (100%) | 12/15 (80%) |
| AR_2B | GCTCTCCGGGTGGCACTCAG | 2 | 4/4 (100%) | 8/8 (100%) |
| AR_2A + AR_2B | GCACCTCGAAAGGTCTTGGA & GCTCTCCGGGTGGCACTCAG | 2 | – | 15/18 (83%) |
| AR_5 | ATGTGACACTGTCAGCTTCT | 5 | 4/7 (57%) | 9/13 (69%) |
aMutation rate was calculated as proportion of blastocysts with indels after microinjection of in vivo produced zygotes or electroporation of in vitro produced zygotes.