Table 3.
PCR primers used to amplify mRNA transcripts.
| Primer name | Product size (bp) | Sequence ID | Efficiency (R2) | Sequence (5′→3′)a |
|---|---|---|---|---|
|
AR_108F AR_108R |
108 | KU705631.1 | 107% (0.99) |
TACCTGTGTGCCAGCAGAAAT AGCTCCCAGTGTCATCCCT |
|
GAPDH_F GAPDH_R |
104 | KJ786424.1 | 92% (0.99) |
GGTGAAGGTCGGAGTGAACG TGAAGGGGTCATTGATGGCG |
|
U2_F U2_R |
105 |
ENSSSCG000 00042922 |
105% (0.96) |
GCCTTTTGGCTAAGATCAAGTGT GGCTTTCTATACCTTCCACAATCT |
|
CYP17A_F CYP17A_R |
132 | AH003260.2 | 109% (0.99) |
GTGCTCTTGGTTTTCTTCTTGCT ACGTCTGGGTAGGAATGGC |
|
AR_RNAseqF AR_RNAseqR |
357 | KU705631.1 |
GTGTGGAGGCATTGGAGCAT AGAGGAAAGTTGTAGTAGTCGTTC |
aPrimer concentrations were 75 nM for AR, 50 nM for GAPDH, 200 nm for U2, and 400 nM for CYP17A. The 357 bp product was a product of RTPCR used for sequencing while other primers were used in qPCR reactions.