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. 2023 Sep 12;14:5610. doi: 10.1038/s41467-023-41455-8

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — Forest plots of selected DEGs (FDR P < 0.1) in opioid-exposed VM (x axis log fold change compared to control, y-axis VM bulk tissue and single nuclei by cell-type RNA-seq) that are linked to alcohol and caffeine consumption, and smoking in PhenomeX database. Bars represent 95% confidence interval (LFC + /−1.96 S.E.). * bold font marks P value from DESeq2 (Wald test following chi-squared distribution with 1 degree of freedom), FDR (Benjamini–Hochberg) P < 0.1. See also Data S7. N = 95 samples (brain donors). Source data are provided as a Source Data file.