Skip to main content
. 2023 Sep 12;14(9):602. doi: 10.1038/s41419-023-06130-2

Fig. 3. Bure regulates POSH-dependent Ex ubiquitination and degradation.

Fig. 3

A Fluorescent micrographs of wing pouch region with gene expression under the control of dpp promoter stained for Ex expression. Quantification of relative Ex signal intensity ratio of GFP+ region/GFP region (A’), n = 6, 6, 6, 6, 6, 6, 16, 13 (from left to right). B S2 cells transfected with HA-tagged POSH and Myc-tagged Ex were treated with or without dsRNAs of Bruce, and the lysates were analyzed by Western blotting. C Bruce is required for POSH-induced Ex ubiquitination in S2 cells. Mean ± SD; n.s. not significant; ****p < 0.0001; ordinary one-way ANOVA (A’). Scale bars: 20 μm for (A).