Reagent or resource	Source	Identifier
Antibodies
mouse monoclonal anti-β-gal	Developmental Studies Hybridoma Bank	Cat# 40-1a; RRID: AB_2314509
rat monoclonal anti-Ci	Developmental Studies Hybridoma Bank	Cat# 2A1; RRID: AB_2109711
rabbit polyclonal anti-Phospho-Histone H3	Cell Signaling Technology	Cat# 9701; RRID: AB_331535
rabbit polyclonal anti- Cleaved Drosophila Dcp-1	Cell Signaling Technology	Cat# 9578; RRID: AB_2721060
HA-Tag (C29F4) Rabbit	Cell Signaling Technology	Cat# 3724; RRID: AB_1549585
Myc-tag mouse monoclonal	Cell Signaling Technology	Cat# 2276; RRID: AB_331783
mouse monoclonal anti-β-actin	Cell Signaling Technology	Cat# 4968, RRID: AB_2313904
guinea-pig monoclonal anti-Ex	Gift from Richard Fehon (University of Chicago)	Maitra et al. [46]
Ubiquitin Antibody Rabbit	Cell Signaling Technology	Cat# 3933, RRID: AB_2180538
Goat anti-Mouse IgG (H + L) Alexa FluorTM Plus 555	Invitrogen	Cat# A32727; RRID: AB_2633276
Goat anti-Rabbit IgG (H + L) Alexa FluorTM Plus 555	Invitrogen	Cat# A32732; RRID: AB_2633281
Chemicals, peptides, and recombinant proteins
Trizol	Thermo Fisher Scientific	Cat# 15596026
Antifade Mounting Medium with DAPI	Vector Laboratories	Cat# H-1800
Critical commercial assays
ESF 921 Insect Cell Culture Medium	Expression System	Cat# 96-001-01
Fetal bovine Serum	CellMax	Cat# SA112
Penicillin-Streptomycin (100X)	Thermo Fisher Scientific	Cat# 15070063
Antibiotic-Antimycotic	Thermo Fisher Scientific	Cat# 15240096
Effectene Transfection Reagent	QIAGEN	Cat# 301427
Protease inhibitor cocktail	Sigma	Cat# P8340
BCA Protein Quantification Kit	Vazyme	Cat# E112-01
PVDF membrane	Merck Millipore	Cat# IPVH00010
Hyperactive Universal CUT&Tag Assay Kit	Vazyme	Cat# TD903-00
Taq Pro Universal SYBR qPCR Master Mix	Vazyme	Cat# Q712-02
MEGAscriptTM RNAi Kit	Invitrogen	Cat# AM1626
Pierce™ Protein A/G Magnetic Beads	Thermo Fisher Scientific	Cat# 88802
FastPure Plasmid Mini Kit	Vazyme	Cat# DC201-01
HiScript II 1st Strand cDNA Synthesis Kit	Vazyme	Cat# R211-01
Oligonucleotides
qPCR for Bruce F	GGGTGACTTTCTCGCTGACA	N/A
qPCR for Bruce R	CCCAGCGAGCTGCTGATTTA	N/A
qPCR for CUT&Tag Bruce F	TTTAAGCGGAATTATCGCG	N/A
qPCR for CUT&Tag Bruce R	CCAGTTATTCAACCGTTAC	N/A
DsRNA for Bruce F	TAATACGACTCACTATAGGGAGAGCATGAAGACCTGTGTGGAT	N/A
Oligonucleotides
DsRNA for Bruce R	CTCCCTATAGTGAGTCGTATTATACAGAACTACTAAAGCTGAGG	N/A
qPCR for rp49 F	CCACCAGTCGGATCGATATGC	N/A
qPCR for rp49 R	CTCTTGAGAACGCAGGCGACC	N/A
qPCR for ex F	GGATCTACCGTAAGCCACCG	N/A
qPCR for ex R	TCATCATGACAGCGGGATCG	N/A
qPCR for diap1 F	AAATGCTTTTTCTGCGGCGT	N/A
qPCR for diap1 R	CTCATCTCCAGCGTCGAGTC	N/A
qPCR for fj F	AGGGATGCGGAAGAATGCAA	N/A
qPCR for fj R	GGCTGAAAGGGCTGTGGTAT	N/A
qPCR for myc F	AGCCAGAGATCCGCAACATC	N/A
qPCR for myc R	CGCGCTGTAGAGATTCGTAGAG	N/A
qPCR for cycE F	GAATGGAGAGCGTACTCCCG	N/A
qPCR for cycE R	TCCAAGAGAATGGCACGCAT	N/A
Experimental models: Cell lines
Drosophila S2 cells	Gift from Jose’ C. Pastor-Pareja (Tsinghua University)	FBtc9000001
Experimental models: Organisms/strains
The Drosophila strains used in this study are listed in Supplementary Table 1	N/A	N/A
Software and algorithms
GraphPad Prism 8.0	GraphPad Software	RRID: SCR_002798
Fiji/ImageJ software	https://fiji.sc	Schindelin et al. [47]
Adobe Photoshop	https://www.adobe.com/cn/products/photoshop.	RRID: SCR_014199