Skip to main content
. 2023 Jun 18;22(9):e13904. doi: 10.1111/acel.13904

FIGURE 1.

FIGURE 1

Deletion of Ido1 or Qprt decreased ovarian nicotinamide adenine dinucleotide (NAD+) levels and reduced mouse fertility. (a) Representative images of immunofluorescence staining of IDO1 (Left panel, green) and QPRT (right panel, green) and nuclei (blue) in ovarian tissue sections. Scale bar, 50 μm. (b) The schematic diagram of Ido1 or Qprt knockout mouse construction by CRISP/Cas 9. (c) Representative Sanger sequence results of the Ido1 (up panel) and Qprt (down panel) Knockout mice. (d) Western blot confirmed the protein expression in ovaries from WT, Ido1 and Qprt Knockout mice. (e) Detection of ovarian NAD+ levels in WT, Ido1 −/− and Qprt −/− mice at different ages using the cycling assay (n = 3 mice for each age). (f) The average litter size was evaluated in WT, Ido1 −/− and Qprt −/− mice at different ages. (g) Cumulative numbers of pups for each female during the defined time period (n = 3 mice for each genotype). (h) The estruses cyclicity in 8‐month‐old WT, Ido1 −/− and Qprt −/− mice (n = 5 mice for each age). *p < 0.05, **p < 0.01, ***p < 0.001.