FIGURE 1.

Confirmation of transgenic mouse models. Polymerase chain reaction (PCR) was used to confirm the deletion of Aldh1a1 and Aldh2 and/or the insertion of human WT‐αSyn (hWT‐αSyn) in WT (lanes 3–4), DKO (lanes 5–6), ASO (lanes 7–8), and DKO/ASO (lanes 9–10) mice. A no template control (NTC; lane 1) and C57Bl/6 lysate (C57; lane 2) were used as controls. The DNA agarose gel is a representation of the Aldh1a1 (top panel; 250 bp), Aldh2 (middle panel; 350 bp), and the Thy1‐αSyn (bottom panel; 349 bp). PCR reactions were used to obtain the corresponding DNA transcripts amplified from tail snips.