Table 1.

Primer sequences for evaluation of the expression levels of biofilm formation genes

Gene	Primer sequence	Tm (°C)	References
luxS-F	5ʹ-AGT GAT GCC GGA ACG CGG-3ʹ	60	[7]
luxS-R	5ʹ-CGG TGT ACC AAT CAG GCT C-3ʹ	60	[7]
mrkA-F	5ʹ-ACG TCT CTA ACT GCC AGG C-3ʹ	60	[7]
mrkA-R	5ʹ-TAG CCC TGT TGT TTG CTG GT-3ʹ	60	[15]
pgaA-F	5ʹ-GCA GAC GCT CTC CTA TGT C-3ʹ	60	[7]
pgaA-R	5ʹ-GCC GAG AGC AGG GGA ATC-3ʹ	60	[7]
wbbM-F	5ʹ-ATG CGG GTG AGA ACA AAC CA-3ʹ	60	[7]
wbbM-R	5ʹ-AGC CGC TAA CGA CAT CTG AC-3ʹ	62	[7]
wzm-F	5ʹ-TGC CAG TTC GGC CAC TAA C-3ʹ	62	[7]
wzm-R	5ʹ-GAC AAC AAT AAC CGG GAT GG-3ʹ	62	[7]
23S rRNA-F	5ʹ-ATC GTA CCC CAA ACC GAC AC-3ʹ	62	[7]
23S rRNA-R	5ʹ-TTC TCC CGA AGT TAC GGC AC-3ʹ	62	[7]

Tm melting temperature