Figure 1.

Evaluation of folate receptor expression and cytotoxic activity of folate-targeted and nontargeted liposomes loaded with panobinostat was evaluated in CLBL-1 cells. (A) Folate receptor expression was evaluated in total cell extracts from CLBL-1 cells using an anti-folate receptor antibody. Loading was controlled with an anti-tubulin antibody. Representative blots are shown. (B) Cells were treated with increasing concentrations of liposomes. After 24 h treatment, cell viability was measured using Alamar Blue reagent. Two replicate wells were utilized to determine each data point and three independent experiments were carried out in different days. Best-fit IC50 values of each formulation were calculated using the log (inhibitor) vs response (variable slope) function. Statistical significance was determined with one-way ANOVA followed by a Tukey's test. Values of p < 0.05 were considered significant. ^****p < 0.0001.