Table 3.

Costs of key consumables and equipment used in this study.

Consumablesb	Unit size	Approx. cost (US$)
Multiplex-PCR master mix	100 reactions	185
De-salted primers (25nmole)	Six pair primer	58
Microcentrifuge tube (0.2 µl)	500 pieces	24
Microcentrifuge tube (1.5 ml)	500 pieces	10
6× DNA loading dye	1 ml	10
DNA ladder, 100 bp	500 µl	35
10× TE buffer, pH 8.0	500 ml	25
10× TAE buffer, pH 8.3	500 ml	12
Agarose, low EEO	100 g	50
Ethidium Bromide	5 g	36
Sodium hydroxide pellet	500 g	4
Sodium citrate tribasic dihydtare	250 g	8
Tris-HCl	100 g	24
Gram-stain reagent	100 reactions	35
Total cost		526
Equipmentc	Quantity	Approx. cost (US$)
Thermal cycler	One	3,638
PCR workstation	One	485
Centrifuge	Two	365
Vortex	One	122
Micropipettes	Five	365
Water-bath	One	245
UV-transilluminator	One	250
Horizontal gel electrophoresis apparatus and power pack	One	1,212
Freezer (−20° C)	One	300
Total cost		6,982

^aCalculating 1 US$ = 82 INR. as of November 2022.

^bConsumable cost US$7 per sample.

^cEquipment cost US$7,000.