Figure 3.

Oxytocin is an enterocytic hormone. a) copy number (CN) of CHGA and OXT transcripts by qPCR and b) percent chromogranin A and oxytocin positive cells measured by flow cytometry in uninduced and induced J2-NGN3 HIOs. For a, points represent averaged triplicate qPCR data each from a separate pooled batch of three 3D organoid wells, and where product could not be amplified, a GAPDH CN normalized value of 1e-5 was used. Only p values < 0.05 are shown, which were determined from the least squares means derived from a linear model with pairwise comparisons corrected using a Benjamini-Hochberg multiple testing correction (see Supplemental Tables S2 and S3). UMAP of Gut Cell Atlas adult jejunal epithelium scRNA-Seq data³² labeled with c) oxytocin counts or d) identified cell clusters (see Supplemental Figure S3J and Supplemental Table S6). e) oxytocin (red) co-stained with enterocyte markers OAT, MTTP, SI, ALDOB, or APOA (green) in 6 μm sectioned human small intestinal tissue. Nuclei stained with DAPI are shown in white. a, b: n = 3 HIO batches with three or more replicate wells combined; c, d: n = 2,791 cells from 4 different patients.