Fig. 2. Device maintained tissue health and barrier function without nicardipine over 72 h. A) Brightfield image showing maintenance of colon morphology. B) Relative fluorescence of 10 kDa dextran fluorescein in the luminal and serosal effluents from the device from the last 2 h of the experiment. C) Peripherin labeled nerve fibers in crypts, mucosal plexus, and myenteric plexus. D) Maintenance of epithelial cells and inner mucosal layer indicated by UEA-1⁺ material. E) Maintenance of immune cells indicated by ACK2⁺ mast cells in lamina propria. F) Maintenance of tight junctions indicated by claudin-1 immunoreactivity. L = lumen, MUC = mucus layer, m = mucosa, sm = submucosa, me = muscularis externa, cr = crypt, scale bars are 50 μm for A, C and E, scale bar is 25 μm for D and F.