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. 2023 Sep 13;155(11):e202213217. doi: 10.1085/jgp.202213217

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© 2023 Akin et al.

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Figure 5. — Sample experiment illustrating how ANO1 knockdown exerted a strong inhibition of 5-HT-induced Ca²⁺ oscillations in a PA from a tamoxifen-injected SMC-ANO1-KO-^ΔEx12-GCaMP3 mouse. The top left panel is an image from a video stack recorded in a pulmonary artery from a conditional smooth muscle cell-specific and inducible ANO1 knockout mouse expressing GCaMP3 specifically in smooth muscle cells, which was exposed to 1 μM 5-HT for 5 min. One ST map constructed from the white line crossing the image is shown in the lower left corner and reveals very little activity. The fluorescence intensity profile as a function of time of two cells from the ST map labeled with the letters a and b are shown on the right. Cell 1 displayed no significant Ca²⁺ activity while Cell 2 showed low-frequency Ca²⁺ events.