Table 4. Laboratory Assays used to detect Zika virus or RNA for infection, dissemination, and transmission assays.

Number of studies (%).

Assay	Description	Infection (n = 45)	Dissemination (n = 40)	Transmission (n = 41)
PCR	Both RT and qRT PCR	10 (22%)	17 (42.5%)	12 (29%)
Infectious virus	Plaque, TCID50 or Fluorescent Focus assay using Vero or C6/36 cells,	16 (36%)	15 (37.5%)	20 (49%)
Combination	Varied protocols, some screening with infectious assay followed by titration with qPCR or PCR screen and confirmation of all or subset of samples by infection assay.	19 (42%)	8 (20.0%)	9 (22%)