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. 2023 Sep 13;9(37):eadi5192. doi: 10.1126/sciadv.adi5192

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Fig. 5. — (A) Hemagglutinin (HA)–tagged SLC7A11 overexpressed in H522 cells was immunoprecipitated followed by Western blotting for OTUB1 and SLC3A2 (left). OTUB1 was immunoprecipitated followed by Western blotting for HA-SLC7A11 and SLC3A2 (right). Western blots of HA-SLC7A11, OTUB1, SLC3A2, and ACTB from total lysates were used as input. (B) GLRX and SLC7A11 Western blots of H522 cells overexpressing GLRX or pcDNA3 empty plasmid. ACTB, loading control. (C) Assessment of GSH levels in cells overexpressing GLRX. (D) Western blots of GLRX and SLC7A11 in H522 cells following small interfering RNA (siRNA)–mediated knockdown of GLRX. ACTB, loading control. (E) GSH levels in H522 cells following knockdown of GLRX. (F) Confirmation of OTUB1 via Western blot analysis in H522 cells following siRNA-mediated knockdown. GAPDH, loading control. (G) GSH levels in H522 cells following knockdown of OTUB1. (H) Nonreducing (nonred) (top) and reducing (bottom) Western blots of SLC7A11 in H522 cells with siRNA-mediated OTUB1 knockdown. ACTB, loading controls. *P < 0.05, **P < 0.01, and ***P < 0.001, ****P < 0.0001.