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. 2023 Aug 31;12:e83466. doi: 10.7554/eLife.83466

Figure 3. Tethering Cα and RIIα to either E1 or Q1 yields differential effects on channel surface density.

Figure 3.

(A) Cartoon showing strategy for surface labeling of BBS-Q1/E1 using BTX-647. (B) Flow cytometry contour plots showing surface channels (BTX-647 fluorescence) and nano expression (CFP fluorescence) in cells expressing BBS-Q1/E1 with nano (left), nanoCα (middle), or nanoRIIα (right). (C) Corresponding cumulative distribution (CDF) histograms of BTX-647 fluorescence. Plot generated from population of CFP-positive cells. (D) Channel surface density (mean BTX-647 fluorescence in CFP-positive cells). *p=0.0003, one-way ANOVA and Tukey HSD post hoc test. (E–H) Cartoon, contour plots, CDF, and average surface labeling of BBS-Q1 in cells expressing BBS-Q1/E1-YFP with nano, nanoCα, or nanoRIIα, same format as A–D. #p<0.05, one-way ANOVA and Tukey HSD post hoc test. (I–L) Cartoon, contour plots, CDF, and normalized average surface labeling of BBS-Q1-YFP in cells expressing BBS-Q1-YFP/E1 with nano, nanoCα, or nanoRIIα, same format as A–D. **p<0.05, one-way ANOVA and Tukey HSD post hoc test.

Figure 3—source data 1. Tethering Cα and RIIα to either E1 or Q1 yields differential effects on channel surface density.