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. 2023 Aug 30;14:1116392. doi: 10.3389/fimmu.2023.1116392

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Copyright © 2023 Ner-Gaon, Peleg, Gazit, Reiner-Benaim and Shay

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Experimentally validated examples of novel differentially spliced genes in the human immune system. (A) LeafCutter cluster representing a skipped exon in FYB1. (B–E) Bar plots of the fraction of JSRs in all samples of all cell types in datasets (B) GSE64655, (C) GSE60424 (D) GSE107011 and (E) GSE115736 are shown. The JSRs of each junction are colored in the color of the arc of that junction in (A). (F) PCR of an independent human sample (G) LeafCutter cluster representing a complex alternative splicing event containing two potentially skipped exons in CD47. (H–K) Bar plots of the fraction of JSRs in all samples of all cell types in datasets (H) GSE64655, (I) GSE60424 (J) GSE107011 and (K) GSE115736 are shown. The JSRs of each junction are colored in the color of the arc of that junction in (A, F) PCR of an independent human sample with the primers marked in (G).