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. 2023 Feb 22;13(8):862–879. doi: 10.1016/j.jpha.2023.02.007

Fig. 1.

Fig. 1

Intracerebral hemorrhage (ICH) induced marked reactive astrogliosis around the lesion core. (A–C) Representative images of glial fibrillary acidic protein (GFAP) immunostaining show the spatiotemporal patterns of astrocytic changes in the ipsilateral hemisphere at 0 (A), 7 (B), and 21 days (C) after ICH. (D) Quantification of GFAP+ area at 0, 7, and 21 days post-injury (dpi). ∗∗∗P < 0.001 vs. sham group; ###P < 0.001 vs. ICH 7 dpi group (n = 3/group). (E) Schematic of experimental approach for astrocyte isolation and RNA sequencing (RNA-seq). (F) Heat map of the reactive astrocyte markers expression (∗fold change > 1.5, P-adj. < 0.05). (G–J) Spatial mapping of astrocyte-specific markers on brain sections (black circle marked the lesion core area). MACS: magnetic-associated cell separation; ACSA-2: anti-astrocyte cell surface antigen 2; Exp: expression; C3: complement C3; Srgn: serglycin; Emp1: epithelial membrane protein 1; Cd14: CD14 molecule.