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. 2023 Feb 22;13(8):862–879. doi: 10.1016/j.jpha.2023.02.007

Fig. 8.

Fig. 8

Delayed microglial depletion partly reversed the functional changes of astrocytic scar. (A) Clustering analysis identified representative cluster of astrocytic scar at 7 days post-injury (dpi). (B) Clustering analysis identified representative cluster of astrocytic scar at 21 dpi. Heatmap of enriched Gene Ontology (GO) terms (biological process) in astrocytic scar at 7 and 21 dpi: (C) GO terms associated tissue damage and (D) GO terms associated with tissue repair. (E) Spatial transcriptomics (ST) analysis showed complement C3 (C3) messenger RNA (mRNA) expression in different clusters at 7 and 21 dpi. (F) Astrocyte-specific RNA sequencing (RNA-seq) showed C3 mRNA expression in astrocyte at 7 and 21 dpi. (G) Scheme of delayed microglial depletion. (H) Quantification of lesion area at 21 dpi. ns: no significance. (I) Reverse transcription quantitative real-time polymerase chain reaction (RT-qPCR) analysis of C3 mRNA expression in astrocytes at 21 dpi. ∗P < 0.05 vs. intracerebral hemorrhage (ICH) + control diet group (n = 8/group). Exp: expression; GFAP: glial fibrillary acidic protein; t-SNE: t-distributed stochastic neighbor embedding; AAV: adeno-associated virus; IGF1: insulin like growth factor 1; OPN: osteopontin.