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. 2023 Jan 7;47(5):627–637. doi: 10.1016/j.jgr.2023.01.004

Fig. 2.

Fig. 2

Suppression of M1 macrophage polarization, pro-inflammatory cytokines and induction of anti-inflammatory cytokine in M1 polarization and in vitro uptake of GENs by Caco2 and RAW 264.7 cells. (A) RAW 264.7 cells were used and proliferated into M1 macrophage with 0.1 μg/mL of LPS. After treatment of GENs from 1 to 50 μg/mL, the gene expression of IL-6 was analyzed. (B) Cell population of F4/80+CD206+ induced by treatment of GENs. (C) M1 and M2 macrophage subtypes induced by GENs. (D,E,F) In M1 macrophage, the gene expression of proinflammatory cytokines including TNF-α and IL-6 and anti-inflammatory cytokine were analyzed. (G) (H) The representative histograms and cellular uptake efficiency of DiD-labeled GENs were analyzed using flow cytometry analysis after 1, 3, 6 hr of incubation. (I) Caco2 cell lines were incubated with DiD-labeled GENs for 6 hr and the image was observed by confocal microscopy. (Blue: Hoechst, Red: DiD-labeled GENs).