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. 2023 Sep 6;621(7978):415–422. doi: 10.1038/s41586-023-06506-6

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© The Author(s) 2023, corrected publication 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Extended Data Fig. 3 — a,b, Representative images of endogenous NG-Polθ foci formation upon (a) laser microirradiation or (b) X-ray irradiation (5 Gy, 1 h) in HEK cells. H3pS10 stains mitotic chromatin. c,d,e,f,g, Representative images and quantification of Polθ foci formation and colocalization with indicated proteins in mitosis following indicated doses of (X-ray) irradiation. In (d), from left to right, n = 88, 90, 76, 81; in (c,f), at least 35; in (g) at least 20 cells were analyzed in each condition in each replicate (two for (f)). h, Quantification of irradiation-induced Polθ foci following transfection with indicated siRNAs. From left to right, n = 57, 38, among two replicates. i, Representative images and quantification of irradiation-induced Polθ foci in WT and BRCA2^−/− cells in indicated cell cycle phases. From left to right, n = 974, 276, 75, 73. Scale bars represent 5 μm. Data represents three biological replicates, except where indicated. Data shows mean +/− S.E.M. For. For (d, i-2 (right)) chi-square test was performed, with chi-square statistics from left to right, in (d): 20.6912, 21.258; in (i-2): 160.6157, 9.7992; for (h) two-tailed Mann-Whitney test; for (g, i-1 (left)) Kruskal-Wallis test, corrected with Dunn’s multiple comparisons test was performed.