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. 2023 Aug 30;621(7978):381–388. doi: 10.1038/s41586-023-06459-w

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — a, Display of a post-mortem human sample (left) of the hippocampus and dlHPC section (middle) that was selected for the iDISCO brain-clearing (right) procedure. b, The iDISCO-cleared section (green) was overlaid to the group average dlHPC (yellow), defined on the basis of its higher number of LH streamlines. c, Staining for MCH⁺ and Alexa Fluor 647 is shown in red and autofluorescence in green within the dlHPC hotspot (high streamline probability with the LH area). The image was acquired using light-sheet microscopy (UltraMicroscope II). Scale bar, 100 μm. 3D visualization is shown in Supplementary Video 1. This 3D histology experiment could not be repeated independently because only a single sample of the human dlHPC was available for the 3D histology experiments at our institution. We therefore approached these data as a unique opportunity for a proof of principle only, testing the feasibility of directly visualizing MCH⁺ LH projections with 3D histology (of which testing was lacking in humans).