Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 Oct 5;621(7978):365–372. doi: 10.1038/s41586-022-05279-8

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Extended Data Fig. 6 — a, Min-max scaled mean expression (log(transcript counts per 10k + 1)) of genes targeted in the single-cell genomic perturbation experiment in neuronal progenitors (NP), intermediate progenitors (IP) and neurons in the primary human and organoid developing cortex, as well as in iPS cells, the embryoid body (EB), ventral telencephalic NPCs, inhibitory neurons of the medial ganglionic eminence (MGE in.), lateral ganglionic eminence (LGE in.), non-telencephalic NPCs, diencephalic excitatory neurons (Dien. ex.) and inhibitory neurons (Dien. in.) and mesencephalic excitatory neurons (Mesen. ex.) and inhibitory neurons (Mesen. in.). b, UMAP embedding coloured by the expression of all targeted genes. The range of expression values is indicated for each feature plot. c, Exemplary Fluorescence-activated cell sorting plots of the sorting scheme used to isolate CROP-seq vector positive iPS cells. d, Phase contrast and CROP-seq vector positive (GFP) imaging during brain organoid development. Images are representative for 48 imaged organoids. Scale Bar is 500 µm.