Extended Data Fig. 4. Two dimensional (MFE-CAI) visualizations of mRNA designs for the Spike protein using human codon preference (a) and yeast codon preference (b) with positive and negative λ’s.

GC% are shown in parentheses. The human genome prefers GC-rich codons that lead to higher CAI designs are with higher GC%, while the yeast genome prefers AU-rich codons that exhibit an opposite relationship between CAI and GC%. See also Fig. 3 for more in silico results of LinearDesign.