TABLE 3.

Prediction performance (r²) of metabolites in the combined cohort of WRAP and Wisconsin ADRC.

NTK biomarkers	N *	Base model r2	Metabolite model r2	Number of input metabolites	Number of elastic net selected metabolites	Elastic net selected metabolites model r2
P-tau	295	0.11	0.71	40	14	0.67
T-tau	296	0.10	0.72	44	19	0.70
Aβ42	296	0.01	0.52	54	47	0.52
Aβ40	293	0.04	0.80	73	71	0.80
NfL	297	0.10	0.24	23	15	0.21
Neurogranin	297	0.07	0.77	51	32	0.76
YKL-40	297	0.25	0.56	15	10	0.56
S100	296	0.05	0.23	20	4	0.19
GFAP	297	0.17	0.35	6	5	0.35
sTREM2	297	0.05	0.53	27	14	0.52
α-synuclein	297	0.03	0.74	90	30	0.67

Variables included in the base model were age, sex, years of education and cohort.

Variables included in the metabolite model were age, sex, years of education, cohort and all replicated significant metabolites for each biomarker.

Variables included in the metabolite model were age, sex, years of education, cohort and elastic net-selected metabolites.

*The sample sizes were different because of the missingness of metabolites.