FIG. 1.

Leishmania mini-exon gene amplification by PCR. Oligonucleotide primers S-1629 (S-29) and S-1630 (S-30) were used for primary PCR. Internal primers MEX-A1 (A1) and MIN-S1 (S1) were used for nested PCR. Lowercase letters indicate noncomplementary bases. The expected DNA fragments of amplified mini-exon gene repeats are indicated by 1n, 2n, and 3n for the primary PCR and by 1n′, 2n′, and 3n′ for the nested PCR. Terminal redundancy of S-1629 and S-1630 may generate PCR products which are 29 nt larger than the genomic repeats (3). Nested PCR may generate PCR products which are 44 nt smaller than those generated by the primary PCR.