FIG. 2.

Sensitivity of mini-exon gene amplification by PCR. DNA samples isolated from L. donovani 2S were serially diluted 10-fold. DNA amounts of approximately 10 ng, 1 ng, 100 pg, 10 pg, 1 pg, 100 fg, 10 fg, and 1 fg (A, lanes 1 to 8, respectively) were subjected to primary PCR. One microliter of each primary PCR product was used as a template DNA for the subsequent nested PCR (B, lanes 1 to 8, respectively). Amplified mini-exon gene repeats are indicated by 1n, 2n, and 3n for the primary PCR and by 1n′, 2n′, and 3n′ for the nested PCR. Lanes M, DNA molecular marker.