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. 2023 Sep 13;13:15131. doi: 10.1038/s41598-023-40763-9

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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(a) Percentage of hemolysis of Ag-Ag₂S@BSA-FA NPs in different concentrations; (b) Cytotoxicity of Ag-Ag₂S@BSA-FA NPs on healthy HFF2 cells; (c) Uptake rate of Ag-Ag₂S@BSA-FA-FITC and Ag-Ag₂S@BSA-FITC NPs by 4T1 cells analyzed by flow cytometry; (d) Cell viability of 4T1 cells under different treatment procedures in the presence and absence of X-ray irradiation (4 Gy) and NPs; (e) 4T1 cells images stained with calcein-AM and PI in the presence and absence of X-ray radiation after different treatments. (Scale bar is 20 µm). Data = mean ± SD; Sign “ns” *, **, ***, and **** show no significant difference, and significant difference with p ≤ 0.05, p ˂ 0.01, p ˂ 0.001 and p ˂ 0.0001, respectively, compared to the control group.