Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2023 Sep 13;14:5650. doi: 10.1038/s41467-023-41171-3

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 3 — The capacity of RVFV-268 and RVFV-140 to neutralize RVFV strain MP-12 was tested on A HEK-293 (human embryonic kidney), B SH-SY5Y (human neuroblastoma), or C HepG2 (human hepatocellular carcinoma) cell lines. Antibody solutions were serially diluted and mixed with 100 infectious units of RVFV strain MP-12 for 1 h at 37 °C. The mixture was added to cells and allowed to incubate for 72 h. Cells were fixed and stained. Foci were counted using a C.T.L. spot counter instrument. The experiment was performed in technical triplicate with three independent experiments. Values are expressed as a percentage of foci in no-antibody controls and mAb CCHF-245 was used as the isotype-matched negative control antibody. The MP-12 neutralization assay was performed twice with three technical replicates in each assay. Results were similar between biological replicates; data shown are the IC₅₀ values of combined data from two independent experiments. IC₅₀ values were calculated using a three-parameter nonlinear fit. Data are presented as mean values +/− SD. Source data are provided as a Source data file.