Fig. 3. Optimization of reaction parameters.

a Relative activities of BAM-mediated OmpT folding in presence of Skp (blue) and SurA (black) as a function of colistin concentration. b Relative activities of pre-folded OmpT (green) as well as BAM-mediated OmpT folding in presence of Skp (blue), SurA (black), or for sonicated OMVs (green), as a function of pH value. c Initial reaction velocities of BAM-mediated OmpT folding as a function of SurA (black dots) and Skp (blue squares) concentrations. d Initial reaction velocities of BAM-mediated OmpT folding at 50 µM Skp plus variable concentrations of SurA. e Initial reaction velocities of BAM-mediated OmpT folding at 15 µM SurA plus variable concentrations of Skp. f Normalized activity at variable DMSO concentration. Initial reaction velocities of BAM-mediated OmpT folding at variable (g) reaction temperature, (h) NaCl, and (i) divalent cation concentrations. For each of panels (d–i), n = 2 independent experiments and data are presented as average values ± standard deviation. The measurements underlying panels (a–i) are shown in Supplementary Figs. 7–9. The data underlying panels (a–i) are provided as Source Data.