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. 2023 Sep 13;6(12):e202302067. doi: 10.26508/lsa.202302067

Figure 3. RB loss promotes replication-dependent PARylation.

Figure 3.

(A, B) Representative images and quantification of EdU and PAR intensity per cell for RPE tet-shRB cells with and without doxycycline induction of shRB (shRB). Cells were untreated or treated with 2 mM emetine for 1 h, and/or 10 μM PARG inhibitor for 30 min. All conditions were incubated with EdU for the final 30 min to label actively replicating cells. Scale bar is 20 μm. PAR intensity data are normalized to the EdU-positive, untreated RPE cells. Error bars represent SD and statistical analysis was performed between five independent experimental replicates. (**) P < 0.01; (***) P < 0.001.