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. 2023 Sep 13;6(12):e202302067. doi: 10.26508/lsa.202302067

Figure S5. PARP trapping synergizes with RB loss to compromise cell cycle progression.

Figure S5.

(A, B) Representative images and quantification of EdU foci in mitotic RPE1 and RPE tet-shRB cells with or without 2 μg/ml doxycycline induction of shRB, incubated with 2.5 μM olaparib for 48 h, as indicated (+), and cultured in 10 μM EdU for 30 min before fixation. Scale bar is 5 μm. Error bars represent the SD and two-way ANOVA and Tukey’s multiple comparisons test. (***) P < 0.001; (ns) nonsignificant P > 0.05, performed between three independent experimental replicates. (C, D) Representative still frames and quantification of cell cycle progression from live cell imaging of control (siScr) and RB-depleted (siRB) RPE FUCCI cells incubated with 2.5 μM olaparib as indicated. Cells were traced from the end of the first G1-phase until the end of the second G1-phase or until the movie ended (*). (C) shows a representative RB-depleted, olaparib-treated cell. Timestamps indicate time after S phase entry. Scale bar is 20 μm.