TABLE 1.
No. of specimens | Culture result | DFA assay result | PCR result | OIA result | No. of specimens positive by two-test minimum criterion |
---|---|---|---|---|---|
20 | + | + | + | + | 20 |
2 | + | + | + | − | 2 |
3 | + | + | − | + | 3 |
5 | + | − | + | + | 5 |
9 | + | − | + | − | 9 |
3 | − | − | + | + | 3 |
10b | − | − | + | − | 0 |
1 | − | + | − | − | 0 |
253 | − | − | − | − | 0 |
Of 306 specimens tested, 39 were positive by culture, 26 were positive by DFA assay, 49 were positive by PCR, and 31 were positive by OIA. Forty-two specimens met the criterion of positivity by a minimum of two tests.
The culture transport media for 7 of 10 specimens which were only PCR positive were subsequently shown to contain C. trachomatis EBs by a modified DFA technique.