Figure 2.

HeLa cell lysate digested with eight commercially available trypsins in a 1:50 E:P ratio. (A) Mean identified number of peptides (gray) and proteins (turquoise) out of four digestion replicates. Error bars indicate standard error (SE). No significant differences (<0.05) based on multiple t-test corrected for multiple testing by Bonferroni. (B) Percentage of missed cleavages in peptides identified in ≥3 replicates. 0 (light gray), 1 (medium gray), and 2 (dark gray) missed cleavages for each trypsin shown.