Figure 3.

Human plasma digested with eight commercially available trypsins in a 1:20 (gray) and 1:50 (purple) E:P ratio. (A) Mean identified number of peptides out of three digestion replicates. (B) Mean identified number of proteins out of three digestion replicates. A–B Error bars indicate SE. Significant differences based on t-test corrected for multiple testing by Bonferroni are shown (*p < 0.05, **p < 0.01, and ***p < 0.0001). (C) Peptides only identified by the respective trypsin (≥50% of replicates) and not present in other trypsins. (D) Percentage of missed cleavages in peptides identified in ≥50% of replicates. 0 (light gray), 1 (medium gray), and 2 (dark gray) missed cleavages for each trypsin shown.