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Quantitative variation of absolute quantified peptides based on ratio to standard to SIS-PrESTs related to commercially available trypsins and a 1:20 (gray) and 1:50 (purple) E:P ratio. (A) CV of 223 peptides quantified in quadruplicates. (B) Pearson correlation of log2 ratio to standard between 0.1 and 10 of 223 peptides identified in eight trypsins and two trypsin concentrations.
