Figure 1.

Schematic of the RPA-CRISPR/Cas12-SPM-AI detection system. The nucleic acids of animal-derived samples are released by PINDBK. Target DNA of the virus is amplified and recognized specifically by the RPA-CRISPR/Cas12a system. The Cas12a–crRNA complex binds to the target DNA, which triggers the collateral cleavage of Cas12a on the reporter (FQ-labeled ssDNA probe). The fluorophore on the reporter is then released, and the fluorescence is detected by the developed SPM. Three different deep learning models, including AlexNet, DenseNet-121, and EfficientNet-B7, with transfer learning, are used to classify the captured fluorescence images.