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. 2023 Sep 14;33:23. doi: 10.1186/s12610-023-00197-9

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 1 — Images of sperm chromatin dispersion test are assessed from halo types: normal group includes (A) big halo spermatozoa (halo thickness equal to or larger than the length of the minor diameter of the core) and (B) medium halo spermatozoa (thickness less than the length of the minor diameter of the core and larger than 1/3 of the minor diameter of the core); abnormal group includes (C) small halo spermatozoa (thickness equal to or less than 1/3 diameter of the minor diameter of the core), (D) without halo spermatozoa, and (E) degraded spermatozoa (sperm show no halo and present a core irregularly or weakly stained, represent a subpopulation of spermatozoa with extensive DNA and nuclear protein damage)