PCR results of dog 2 (the only dog that remained PCR positive posttreatment) before treatment and after 42 days of doxycycline (10 mg/kg per os q24h) treatment. S, splenic aspirate; M, bone marrow aspirate; B, blood sample; P, positive control retrieved from E. canis-infected DH82 cells; N, negative control retrieved from noninfected DH82 cells.